RNA Synthesis Optimization Analysis

Visualizing and interpreting RNA yield data to optimize synthesis protocols

Highest Yield

2.027 µg/µL

Achieved on 9/11/2025 with 1.5µg DNA

Average Yield

0.492 µg/µL

Across all successful experiments

Optimal DNA Input

1.5µg

Consistently produced highest yields

RNA Yield Over Time

DNA Input vs RNA Yield

Experimental Data

Date Sample DNA (µg) A260 A260/280 RNA (µg/µL)

Key Findings & Recommendations

1. Optimal DNA Input

The data clearly shows that 1.5µg of DNA template consistently produces the highest RNA yields (average 0.971µg/µL) compared to lower inputs.

2. Time Course Analysis

The 4-hour time point (7/25/2025) showed significantly lower yield (0.0408µg/µL) compared to standard incubations, suggesting longer reaction times are needed.

3. Extraction Method Impact

Phenol:chlorform extraction showed variable results, with one sample (1.5µg input) producing excellent yield (1.5528µg/µL) while others were lower.

4. Protocol Recommendations

  • Use 1.5µg DNA template for consistent high yields
  • Standardize reaction time to ≥16 hours
  • Evaluate alternative purification methods for consistency
  • Monitor A260/280 ratios for quality control (ideal range 1.8-2.1)